Current State of Method Development for PCR Sample Peparations

The sensitivity, specificity and reproducibility of molecular genetic methods analysis largely depend on the quality preliminary preparation analyzed samples. During sample preparation, tasks disinfecting pathogenic material, lysing cell membranes, removing compounds impurities that inhibit polymerase chain reaction (PCR), as well concentrating nucleic acids are solved. purpose this work is to select modern approaches for PCR. Among variety different most widespread based chemical lysis membranes using chaotropic compounds, followed by purification solid-phase extraction magnetic particles. This approach implemented both in commercial kits manual various automated systems isolation acids. commercially available stations shows their technical characteristics similar: duration one cycle 40–90 minutes; volume samples from 0.1 2.0 ml; number simultaneously processed max – 96, min 8. method acid main differences type samples, technologies test material DNA extraction. Our experience use particle acids, stationary field laboratories confirms effectiveness reliability technology. Further development improvement hardware such will, obviously, be aimed at miniaturizing equipment, developing portable automatic stations, integrating process PCR device